Cloning and Sequencing of the Beta-glucosidase Gene from Acetobacter xylinum ATCC 23769

The relationship between cellulase activity and oligosaccharides and cellulose productions by Acetobacter xylinum ATCC 23769

We have tried to investigate various carbohydrates production and cellulose degrading enzyme activity in the culture broth of cellulose producing microorganism, Acetohacter xylinum to clarify the role of cellulase for cellulose production. Carboxymethylcellulose (CMC) degrading activity and various oligosaccharides in addition to cellulose have already been detected after one-day culture. These...

Purification, characterization, gene cloning, and sequencing of a new beta-glucosidase from Bacillus circulans subsp. alkalophilus.

An intracellular beta-glucosidase was purified from cell extracts of Bacillus circulans subsp. alkalophilus by NAD affinity and high-performance anion-exchange chromatographies. The enzyme was active against a wide range of aryl-beta-glucosides and beta-linked disaccharides. The structural gene for beta-glucosidase was cloned in Escherichia coli. The beta-glucosidase gene consisted of an open r...

IDENTIFICATION, ISOLATION, CLONING AND SEQUENCING APARTIALANNEXIN GENE FROM AUREOBASIDIUM PULLULANS

Background and Objectives: Annexin is the common name for genes and proteins that were identified as calcium-dependent phospholipid-binding proteins. Recently a more complex set of functions has been recognized for this superfamily of proteins including in vesicle trafficking, cell division, apoptosis, calcium signalling, mineralization, crystal nucleation inside the extracellular organelle...

Survey on Nano-fibers from Acetobacter Xylinum

تولید و تعیین مشخصات سلولز باکتریایی با استفاده از باکتری Acetobacter xylinum ATCC 10245

سابقه و هدف: سلولز باکتریایی با الیافی حدود 100 مرتبه کوچک‌تر از سلولز گیاهی دارای خصوصیاتی چون الاستیسیته زیاد، استحکام مرطوب بالا و قابلیت تطبیق‌پذیری می‌باشد. یکی از کاربردهای عمده آن در مهندسی بافت به‌عنوان ماتریس خارج سلولی سه بعدی برای پیوسته نگه داشتن سلول‌ها و کنترل ساختار بافت و تنظیم رفتار سلولی است. این تحقیق با هدف تولید سلولز باکتریایی و بررسی مشخصات آن صورت گرفت. مواد و روش‌ها: ...